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Process Associate - Cell Culture and Purification
Bethesda, MD
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Workforce Genetics is currently seeking an experienced Process Associate specializing in Cell Culture & Purification. 


The core responsibilities will be focused on three key areas:


Cell Culture (Upstream Processing):


• Perform mammalian cell culture operations, including cell line maintenance, seed train expansion, and bioreactor operations (shake flasks, wave bags, and stirred-tank bioreactors).

• Optimize growth conditions and process parameters to improve cell viability and productivity.

• Assist in media preparation, feeding strategies, and metabolite analysis.

• Monitor and document process parameters to ensure consistency and scalability.


Purification (Downstream Processing):


• Perform protein purification using chromatography techniques (affinity, ion exchange, hydrophobic interaction, size exclusion) and filtration methods.

• Optimize purification processes for yield, purity, and scalability.

• Support process development, scale-up, and technology transfer activities.

• Conduct analytical characterization of purified proteins, including SDS-PAGE, Western blot, and UV-Vis spectroscopy.


Documentation and QA:


• Maintain accurate records in lab notebooks and prepare technical reports.

• Ensure compliance with GLP/GMP guidelines, safety protocols, and regulatory standards.

• Support equipment maintenance, calibration, and troubleshooting.

• Collaborate with cross-functional teams in bioprocess development and manufacturing.


Qualifications and Experience:

 

• Bachelor’s or Master’s degree in Biochemistry, Biotechnology, Chemical Engineering, or a related field.

• 1 + years of experience in cell culture, protein purification, or related bioprocessing.

• Hands-on experience with mammalian cell culture techniques and bioreactor operations.

• Knowledge of protein purification techniques and chromatography principles.

• Experience with monoclonal antibody production and ADC manufacturing.

• Familiarity with process development, scale-up, and technology transfer.

• Experience with process analytical tools (e.g., Bioanalyzer, HPLC, Octet) for protein characterization.

• Understanding of GMP/GLP compliance and regulatory guidelines.




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